<i>In vitro</i>generation of disease-specific autoantibodies produced by B cells from patients with NMOSD and MOGAD.

Abstract The main pathogenic feature of neuromyelitis optica spectrum disorder (NMOSD) and myelin oligodendrocyte glycoprotein associated disease (MOGAD) is the presence autoantibodies against water channel, aquaporin-4 (AQP4) (MOG), respectively. However, underlying mechanisms in which B cells from these patients produce largely unknown. Our study aims to establish a human culture system that could characterize contribution production autoantibodies. PBMCs were obtained NMOSD (n=30) MOG (n=10) patients. differentiated into plasmablasts vitrowith different stimuli resembling mechanism related with MOGAD development. After 6d culture, cell proliferation plasmablast frequency assessed. Quantification total IgG levels detection AQP4-IgG or MOG-IgG determined on D13. data showed R848 (TLR7/8), regardless sCD40L cytokines, resulted (ave: 78.2% ± 18.5 SEM) 90.4% 5.2 SEM). Moreover, vitrogeneration require 45.6 21.1 54.4% 11.5 Further until D13 secretion by generated 18,150.07 ng/mL) 17,959.25 ng/mL). For all conditions plasmablasts, detected be reactive either AQP4-transfected (10/30 patients) MOG-transfected (6/10 patients). This vitromodel have important implications for therapy selection understanding was supported International Cooperation Education Program National Cancer Center Korea (NCCRI·NCCI 52210-52211).